The wild soybean, the direct ancestor of the soybean, is distributed mainly in eastern China, eastern Russia, Korea and Japan ( Lu, 2004). The major production areas are the United States of America, Brazil, Argentina, China, Paraguay, India and Canada. The production of soybean ranks fourth to rice, wheat and maize in terms of world crop production. The soybean is one of the most important leguminous crops in the world and is used for edible proteins, oils, fodder and in various processed foods. The genetic factors and the metabolites on Chr20 in Peking will be useful for understanding mechanisms underlying CCW resistance and breeding resistant soybean cultivars. Moreover, a line possessing a Peking genomic segment on Chr20 inhibited growth of the CCW. The genomic segments of Peking on chromosome 06 (Chr06) and Chr20 had a clear association with these metabolites. The structure of seven metabolites were determined by nuclear magnetic resonance (NMR) analysis. Several characteristic metabolites for Peking were identified by the metabolomic approach using liquid chromatography/mass spectrometry combined with a principle component analysis. We found a resistance to CCW in Peking by the detached-leaf feeding assay, and subsequently determined the genetic and metabolic basis of the resistance mechanism using chromosome segment substitution lines (CSSLs) of Peking. In this study, we focused on a primitive soybean landrace, Peking, which has retained resistances to various pests. Thus, understanding the genetic and metabolic resistance mechanisms of CCW as well as integrating other new resistance genes are required. Although quantitative trail loci related to CCW resistance have been introduced into leading soybean cultivars, these do not exhibit sufficient resistance against CCW. The common cutworm (CCW Spodoptera litura) is one of the major insect pests of soybean in Asia and Oceania. 3Division of Agronomy and Horticultural Science, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.2Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.1Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba, Japan.Ryu Nakata 1†, Mariko Yano 2, Susumu Hiraga 1, Masayoshi Teraishi 3, Yutaka Okumoto 3†, Naoki Mori 2 and Akito Kaga 1*
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